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ADVANCED
CELL SORTING PLATFORM

CELLOPTIMISER
PLATFORM

01 

CellOptimiser is an electro-optical system for large-scale sorting of multiplicity of adherent and non-adherent cells as well as embryoid bodies. From the beginning it was designed and built as a highly scalable bio-processing device with the focus on cell manufacturing. 

02 

CellOptimiser is a fluorescence- or morphology-directed cell culture-sorting system that simultaneously measures optical properties and processes biological cells non-invasively, with ultra-high speed and precision. For additional flexibility, optical subsystems (reflected image and epi-luminescent microscopy) may 

03 

CellOptimiser processes own sterile, disposable cartridges - as substrates for the adherent cell culture.

04 

CellOptimiser is equiped with optional microfluidics module - µFluidics,  which is capable for sorting of suspended cells, is an option to the basic system. Massive parallel architecture of the microfluidics module allows for flow sorting with very high speeds. It permits use of CellOptimiser as a direct replacement to the latest-generation capillary-based FACS platforms at sorting, enrichment or test and quality control operations.

GLOBAL  SERVICES

R&D Services

  • Leverage unique NeuroSyntek tools and methodologies  

  • Establish and verify pre- & clinical protocols 

  • Design for manufacturing, simulation, modeling

  • Intellectual Property secure, flexible and innovative environment

Clinical Services

  • Leverage unique NeuroSyntek tools and methodologies  

  • Execute protocols for clinical trials with predictable outputs in delivery and quality

  • Scalable for large volume therapies

RESULTS  WITH  PARTNERS

with Stanford Medicine (above): selective CellOptimiser EGFP-mRNA fibroblast transfection by Light-Induced Electroporation (LEP). A: cell selection; B: LEP; C: cell after LEP. Lower voltages (1-3V) vs. other transfection systems (1-2kV) and lower mRNA amounts. D1-D2: images of after-transfection cells

(below): images of reprogrammed iPSCs (from fibroblast cells) on CellOptimiser-chips; (A) reflected light; (B) fluorescent light; (C) control

with Massachusetts General Hospital: C2C12 murine myoblasts; attached to the surface of micro-patterned NeuroSyntek

chips (left: control)

with Yale Systems Biology Institute: attached onto NeuroSyntek chips: (left) Human Endothelial Cells (with green florescent in cell cytoplasm to image cells); (right) Human Pericytes (with red fluorescent actin filament stain to image cells). 

For cancer screening & toxicology applications 

NeuroSyntek

bio-Neural Networks

HIGH-SPEED SORTING

HEMATOPOIETIC & CAR-T CELLS

  • Closed and disposable 3-chamber cell cartridge

  • Sterile packaged

  • Automatic alignment with optical system

  • Cells are processed in forward-reverse flow pattern -> allows multi-pass sorting without cartridge change

  • Miniaturized fluorescent-assisted droplet fluidic cell sorter

  • Scalable silicon-based architecture

  • High-speed cell suspension sorting

  • Direct replacement of capillary-based FACS platform

  • Designed to be integrated with NeuroSyntek CallOptimiser platform

Dr. David DiGiusto

​

* Director of Stem Cell & Cellular  Therapeutics, Stanford Hospitals & Clinics

* North American Vice President Elect of ISCT

 * Molecular-Cell Biology, PhD, Univ. of Colorado

Dr. Fumiaki Ikeno

​

* Board, US-Japan Council

 * Program Director, Stanford Biodesign Center    

* Chief Medical Officer (MedVenture Partners, Tokyo-Silicon Valley)

Professor Andre Levchenko

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* Director of Yale Systems Biology Institute

* Director of Cancer Systems Biology@Yale (CaSB@Yale)

 * Professor of Biomedical Engineering

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